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rat syntaxin3  (Addgene inc)


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    Addgene inc rat syntaxin3
    VAMP2-bearing donor liposomes were incubated 3 h on ice with acceptor proteoliposomes as specified, with or without Munc18a. Each sample was then aliquoted for clustering and lipid-mixing assays. A) Cumulative distribution plots show the results of the clustering assay. B) Following the lipid-mixing assay, the mean values of the maximal lipid-mixing rates are presented and error bars indicate SD (n=4). Student’s t-test was used to assess the significance of the difference between the “– Munc18a” results and the “+ Munc18” results. * indicates P < 0.05 whereas ** indicates P < 0.01. Note that the extent of Munc18a-dependent acceleration of lipid mixing does not necessarily correlate linearly with the extent of change in clustering, which implies either the insufficient resolution of the clustering assay or possibly different modes of action exploited by Munc18a in clustering and lipid mixing respectively. C) To determine the biochemical interaction between Munc18a and Q-SNAREs, His6-Munc18a was incubated with specified proteoliposomes at 4°C overnight before Ni-NTA agarose was added to bring down His6-Munc18a and associating molecules. Eluates were subject to SDS-PAGE followed by Coomassie Brilliant Blue staining. The top and bottom panels are from two separate gels. D) Densitometry was performed and the intensity of the syntaxin bands in the pull-down was normalized for the input. The values of the wild-type <t>syntaxin3</t> and syntaxin4 were set as 100% respectively and then used to calculate the relative levels of mutant syntaxins in the pull-down. Student’s t-test was used to assess the significance of the difference between the “WT” results and the “∆N” results. ** indicates P < 0.01. Error bars represent SD (n=3).
    Rat Syntaxin3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat syntaxin3/product/Addgene inc
    Average 88 stars, based on 3 article reviews
    rat syntaxin3 - by Bioz Stars, 2026-04
    88/100 stars

    Images

    1) Product Images from "Munc18a Clusters SNARE-Bearing Liposomes Prior to Trans-SNARE Zippering"

    Article Title: Munc18a Clusters SNARE-Bearing Liposomes Prior to Trans-SNARE Zippering

    Journal: The Biochemical journal

    doi: 10.1042/BCJ20170494

    VAMP2-bearing donor liposomes were incubated 3 h on ice with acceptor proteoliposomes as specified, with or without Munc18a. Each sample was then aliquoted for clustering and lipid-mixing assays. A) Cumulative distribution plots show the results of the clustering assay. B) Following the lipid-mixing assay, the mean values of the maximal lipid-mixing rates are presented and error bars indicate SD (n=4). Student’s t-test was used to assess the significance of the difference between the “– Munc18a” results and the “+ Munc18” results. * indicates P < 0.05 whereas ** indicates P < 0.01. Note that the extent of Munc18a-dependent acceleration of lipid mixing does not necessarily correlate linearly with the extent of change in clustering, which implies either the insufficient resolution of the clustering assay or possibly different modes of action exploited by Munc18a in clustering and lipid mixing respectively. C) To determine the biochemical interaction between Munc18a and Q-SNAREs, His6-Munc18a was incubated with specified proteoliposomes at 4°C overnight before Ni-NTA agarose was added to bring down His6-Munc18a and associating molecules. Eluates were subject to SDS-PAGE followed by Coomassie Brilliant Blue staining. The top and bottom panels are from two separate gels. D) Densitometry was performed and the intensity of the syntaxin bands in the pull-down was normalized for the input. The values of the wild-type syntaxin3 and syntaxin4 were set as 100% respectively and then used to calculate the relative levels of mutant syntaxins in the pull-down. Student’s t-test was used to assess the significance of the difference between the “WT” results and the “∆N” results. ** indicates P < 0.01. Error bars represent SD (n=3).
    Figure Legend Snippet: VAMP2-bearing donor liposomes were incubated 3 h on ice with acceptor proteoliposomes as specified, with or without Munc18a. Each sample was then aliquoted for clustering and lipid-mixing assays. A) Cumulative distribution plots show the results of the clustering assay. B) Following the lipid-mixing assay, the mean values of the maximal lipid-mixing rates are presented and error bars indicate SD (n=4). Student’s t-test was used to assess the significance of the difference between the “– Munc18a” results and the “+ Munc18” results. * indicates P < 0.05 whereas ** indicates P < 0.01. Note that the extent of Munc18a-dependent acceleration of lipid mixing does not necessarily correlate linearly with the extent of change in clustering, which implies either the insufficient resolution of the clustering assay or possibly different modes of action exploited by Munc18a in clustering and lipid mixing respectively. C) To determine the biochemical interaction between Munc18a and Q-SNAREs, His6-Munc18a was incubated with specified proteoliposomes at 4°C overnight before Ni-NTA agarose was added to bring down His6-Munc18a and associating molecules. Eluates were subject to SDS-PAGE followed by Coomassie Brilliant Blue staining. The top and bottom panels are from two separate gels. D) Densitometry was performed and the intensity of the syntaxin bands in the pull-down was normalized for the input. The values of the wild-type syntaxin3 and syntaxin4 were set as 100% respectively and then used to calculate the relative levels of mutant syntaxins in the pull-down. Student’s t-test was used to assess the significance of the difference between the “WT” results and the “∆N” results. ** indicates P < 0.01. Error bars represent SD (n=3).

    Techniques Used: Liposomes, Incubation, SDS Page, Staining, Mutagenesis



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    VAMP2-bearing donor liposomes were incubated 3 h on ice with acceptor proteoliposomes as specified, with or without Munc18a. Each sample was then aliquoted for clustering and lipid-mixing assays. A) Cumulative distribution plots show the results of the clustering assay. B) Following the lipid-mixing assay, the mean values of the maximal lipid-mixing rates are presented and error bars indicate SD (n=4). Student’s t-test was used to assess the significance of the difference between the “– Munc18a” results and the “+ Munc18” results. * indicates P < 0.05 whereas ** indicates P < 0.01. Note that the extent of Munc18a-dependent acceleration of lipid mixing does not necessarily correlate linearly with the extent of change in clustering, which implies either the insufficient resolution of the clustering assay or possibly different modes of action exploited by Munc18a in clustering and lipid mixing respectively. C) To determine the biochemical interaction between Munc18a and Q-SNAREs, His6-Munc18a was incubated with specified proteoliposomes at 4°C overnight before Ni-NTA agarose was added to bring down His6-Munc18a and associating molecules. Eluates were subject to SDS-PAGE followed by Coomassie Brilliant Blue staining. The top and bottom panels are from two separate gels. D) Densitometry was performed and the intensity of the syntaxin bands in the pull-down was normalized for the input. The values of the wild-type <t>syntaxin3</t> and syntaxin4 were set as 100% respectively and then used to calculate the relative levels of mutant syntaxins in the pull-down. Student’s t-test was used to assess the significance of the difference between the “WT” results and the “∆N” results. ** indicates P < 0.01. Error bars represent SD (n=3).
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    rat syntaxin3 - by Bioz Stars, 2026-04
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    VAMP2-bearing donor liposomes were incubated 3 h on ice with acceptor proteoliposomes as specified, with or without Munc18a. Each sample was then aliquoted for clustering and lipid-mixing assays. A) Cumulative distribution plots show the results of the clustering assay. B) Following the lipid-mixing assay, the mean values of the maximal lipid-mixing rates are presented and error bars indicate SD (n=4). Student’s t-test was used to assess the significance of the difference between the “– Munc18a” results and the “+ Munc18” results. * indicates P < 0.05 whereas ** indicates P < 0.01. Note that the extent of Munc18a-dependent acceleration of lipid mixing does not necessarily correlate linearly with the extent of change in clustering, which implies either the insufficient resolution of the clustering assay or possibly different modes of action exploited by Munc18a in clustering and lipid mixing respectively. C) To determine the biochemical interaction between Munc18a and Q-SNAREs, His6-Munc18a was incubated with specified proteoliposomes at 4°C overnight before Ni-NTA agarose was added to bring down His6-Munc18a and associating molecules. Eluates were subject to SDS-PAGE followed by Coomassie Brilliant Blue staining. The top and bottom panels are from two separate gels. D) Densitometry was performed and the intensity of the syntaxin bands in the pull-down was normalized for the input. The values of the wild-type syntaxin3 and syntaxin4 were set as 100% respectively and then used to calculate the relative levels of mutant syntaxins in the pull-down. Student’s t-test was used to assess the significance of the difference between the “WT” results and the “∆N” results. ** indicates P < 0.01. Error bars represent SD (n=3).

    Journal: The Biochemical journal

    Article Title: Munc18a Clusters SNARE-Bearing Liposomes Prior to Trans-SNARE Zippering

    doi: 10.1042/BCJ20170494

    Figure Lengend Snippet: VAMP2-bearing donor liposomes were incubated 3 h on ice with acceptor proteoliposomes as specified, with or without Munc18a. Each sample was then aliquoted for clustering and lipid-mixing assays. A) Cumulative distribution plots show the results of the clustering assay. B) Following the lipid-mixing assay, the mean values of the maximal lipid-mixing rates are presented and error bars indicate SD (n=4). Student’s t-test was used to assess the significance of the difference between the “– Munc18a” results and the “+ Munc18” results. * indicates P < 0.05 whereas ** indicates P < 0.01. Note that the extent of Munc18a-dependent acceleration of lipid mixing does not necessarily correlate linearly with the extent of change in clustering, which implies either the insufficient resolution of the clustering assay or possibly different modes of action exploited by Munc18a in clustering and lipid mixing respectively. C) To determine the biochemical interaction between Munc18a and Q-SNAREs, His6-Munc18a was incubated with specified proteoliposomes at 4°C overnight before Ni-NTA agarose was added to bring down His6-Munc18a and associating molecules. Eluates were subject to SDS-PAGE followed by Coomassie Brilliant Blue staining. The top and bottom panels are from two separate gels. D) Densitometry was performed and the intensity of the syntaxin bands in the pull-down was normalized for the input. The values of the wild-type syntaxin3 and syntaxin4 were set as 100% respectively and then used to calculate the relative levels of mutant syntaxins in the pull-down. Student’s t-test was used to assess the significance of the difference between the “WT” results and the “∆N” results. ** indicates P < 0.01. Error bars represent SD (n=3).

    Article Snippet: The cDNAs for full-length or N-terminally truncated rat syntaxin3 and syntaxin4 were inserted individually into the LIC site of pET MBP His 6 LIC cloning vector (gift from Scott Gradia; Addgene plasmid # 37237) to generate pET-Stx3-TCS(Tev Cleavable Site)-MBP-His 6 , pET-Stx3ΔN-TCS-MBP-His 6 , pET-Stx4-TCS-MBP-His 6 , and pET-Stx4ΔN-TCS-MBP-His 6 .

    Techniques: Liposomes, Incubation, SDS Page, Staining, Mutagenesis